The Microtomist’s Vade-Mecum by Arthur Bolles Lee (1885)

Lee presents an organized collection of 660 tested reagents and procedures for fixing, staining processing, mounting and demonstrating all manners of biological and histotechnological material as known up to the publication date of 1885. As Lee points out in the preface, the book provides "a concise but complete account of all the methods of preparation." The introduction explains the purpose of the book, which is to provide zoological scientists with a collection of microscopical methods to participate in a wide list of needs. It is intended to reveal additional information from the images of nature viewed or enhanced for study through the microscope.

It outlines the evolution of microscopy as a series of solutions to specific problems or needs. Each of the methods described is such a solution. The entire second chapter is devoted to fixing agents. Thirty-nine individual instructions and formulae are given along with a philosophical bases of the work. The next 15 chapters describe a wide variety of stains and staining procedures, each with a unique affinity and purpose. Chapter 18 then discusses hardening agents, with 15 process and formula results. Lee describes the impact of the introduction of paraffin infiltration methods. "Methods of embedding have now been brought to such a degree of perfection that the thorough hardening of soft tissues that was formerly necessary in order to cut thin section from them is now, in the majority of cases, no longer necessary." In Chapter 19, Lee presents the theory of embedding, and comments on the use of the microtome. He goes into great detail in discussing the needs and means to provide fine quality tissue sections of minute thickness. The following chapters include discussions on fusion, congelation, coagulation, and evaporation embedding-masses. Capters are also included on: Serial Section Mounting, Clearing Agents, Examination Media, Cements, and Varnishes, Injection-Masses, Maceration and Corrosions, Decalcification, Descilicification, and Bleaching, Cytological Methods, Embryological Methods, Integument and Tactile Organs of Vertabrata, Retina of Vertabrata, Inner Ear of Vertabrata, Nerve-Endings in Muscle, Medullated Nerve, Myelon, Connective Tissues, Blood, Gland and Lymphatics, Mollusca, Arthropoda, Vermes, Colenterata and Porifera, Protista, and Varia. The utility and appropriateness of many of these prcedures have survived into the most modern 20th century microscopic laboratories. Only 8 left! Price: $65.00


Price: $65.00

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